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Fluid flow shear stress over podocytes is increased in the solitary kidney
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Pediatrics.
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2014 (English)In: Nephrology, Dialysis and Transplantation, ISSN 0931-0509, E-ISSN 1460-2385, Vol. 29, no 1, 65-72 p.Article in journal (Refereed) Published
Abstract [en]

Glomerular hyperfiltration is emerging as the key risk factor for progression of chronic kidney disease (CKD). Podocytes are exposed to fluid flow shear stress (FFSS) caused by the flow of ultrafiltrate within Bowmans space. The mechanism of hyperfiltration-induced podocyte injury is not clear. We postulated that glomerular hyperfiltration in solitary kidney increases FFSS over podocytes. Infant SpragueDawley rats at 5 days of age and C57BL/6J 14-week-old adult mice underwent unilateral nephrectomy. Micropuncture and morphological studies were then performed on 20- and 60-day-old rats. FFSS over podocytes in uninephrectomized rats and mice was calculated using the recently published equation by Friedrich et al. which includes the variablesusingle nephron glomerular filtration rate (SNGFR), filtration fraction (f), glomerular tuft diameter (2R(T)) and width of Bowmans space (s). Glomerular hypertrophy was observed in uninephrectomized rats and mice. Uninephrectomized rats on Day 20 showed a 2.0-fold increase in SNGFR, 1.0-fold increase in 2R(T) and 2.1-fold increase in FFSS, and on Day 60 showed a 1.9-fold increase in SNGFR, 1.3-fold increase in 2R(T) and 1.5-fold increase in FFSS, at all values of modeled s. Similarly, uninephrectomized mice showed a 2- to 3-fold increase in FFSS at all values of modeled SNGFR. FFSS over podocytes is increased in solitary kidneys in both infant rats and adult mice. This increase is a consequence of increased SNGFR. We speculate that increased FFSS caused by reduced nephron number contributes to podocyte injury and promotes the progression of CKD.

Place, publisher, year, edition, pages
2014. Vol. 29, no 1, 65-72 p.
Keyword [en]
fluid flow shear stress, glomerular hyperfiltration, podocyte, solitary kidney
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-220318DOI: 10.1093/ndt/gft387ISI: 000330664000011OAI: oai:DiVA.org:uu-220318DiVA: diva2:705123
Available from: 2014-03-14 Created: 2014-03-12 Last updated: 2014-03-14Bibliographically approved

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Celsi, Gianni E.
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