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Validation of PCR assays for detection of Shiga toxin-producing E. coli O104:H4 and O121 in food
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2014 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Shigatoxin-producing Escherichia coli (STEC) can cause infections in humans which can beserious and sometimes fatal. There is a great need for methods that are able to detect differentserogroups of STEC. In this project, conventional and real-time PCR assays for detection ofSTEC O104:H4 and O121, as recommended by the European Union Reference Laboratory(EU-RL) for STEC, were validated. The specificity, limit of detection, repeatability,efficiency and robustness were determined for three real-time PCR assays. The validationshowed that the real-time PCR reactions were specific and sensitive although some additionaltests are required.

Place, publisher, year, edition, pages
2014. , 35 p.
Keyword [en]
Pathogenic E. coli, STEC, VTEC, EHEC, PCR, validation
National Category
Engineering and Technology
URN: urn:nbn:se:uu:diva-220478OAI: oai:DiVA.org:uu-220478DiVA: diva2:705306
Educational program
Molecular Biotechnology Engineering Programme
Available from: 2014-03-17 Created: 2014-03-15 Last updated: 2014-03-17Bibliographically approved

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