Normal oral keratinocytes and head and neck squamous carcinoma cells induce an innate response in fibroblasts
2016 (English)In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 36, no 5, 2131-2137 p.Article in journal (Other academic) Published
Background: Tumor stroma is similar to the connective tissue of chronic inflammation. The extracellular matrix of tumors is formed by cancer-associated fibroblasts that also modulate the inflammatory response. Materials and Methods: We studied the ability of oral keratinocytes (NOK) and oral squamous cell carcinoma cells (SCC) to induce an innate immune response in fibroblasts. Co-cultures with fibroblasts in collagen gels and keratinocytes in inserts were used. Pentraxin 3 (PTX3) was used as an indicator of an innate immune response. Results: SCC and NOK up-regulated fibroblast mRNA expression and protein release of PTX3. mRNA levels were more pronounced in cultures with malignant cells. The induction of PTX3 was abrogated by an interleukin-1 receptor antagonist Conclusion: Keratinocytes have the capacity to induce an interleukin-1-dependent innate immune response by fibroblasts in vitro. This could be important for subsequent fibroblast modulation of the inflammatory reaction in non-malignant and malignant disease processes.
Place, publisher, year, edition, pages
2016. Vol. 36, no 5, 2131-2137 p.
co-culture, pentraxin-3, extracellular matrix, interleukin-1 alpha, tumor stroma
Surgery Cancer and Oncology
Research subject Plastic Surgery
IdentifiersURN: urn:nbn:se:uu:diva-221123DOI: 000375456200010ISI: 27127114OAI: oai:DiVA.org:uu-221123DiVA: diva2:708072