uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Protein engineering for development of new hydrolytic biocatalysts
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC.
2014 (English)In: Current opinion in chemical biology, ISSN 1367-5931, E-ISSN 1879-0402, Vol. 21, 42-47 p.Article, review/survey (Refereed) Published
Abstract [en]

Hydrolytic enzymes play important roles as biocatalysts in chemical synthesis. The chemical versatility and structurally sturdy features of Candida antarctica lipase B has placed this enzyme as a common utensil in the synthetic tool-box. In addition to catalyzing acyl transfer reactions, a number of promiscuous activities have been described recently. Some of these new enzyme activities have been amplified by mutagenesis. Epoxide hydrolases are of interest due to their potential as catalysts in asymmetric synthesis. This current update discusses recent development in the engineering of lipases and epoxide hydrolases aiming to generate new biocatalysts with refined features as compared to the wild-type enzymes. Reported progress in improvements in reaction atom economy from dynamic kinetic resolution or enantioconvergence are also included.

Place, publisher, year, edition, pages
2014. Vol. 21, 42-47 p.
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:uu:diva-221378DOI: 10.1016/j.cbpa.2014.03.015ISI: 000341900100008OAI: oai:DiVA.org:uu-221378DiVA: diva2:708801
Available from: 2014-03-29 Created: 2014-03-29 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

Accepted_version(3435 kB)538 downloads
File information
File name FULLTEXT03.pdfFile size 3435 kBChecksum SHA-512
7828d9506d6f40d6566ea71321ca80a16870f729bc70da9976528f98da32384cf0b7ea1a3225a6ccd096014df69c79aab513b4e8777755bf1f539f97de7e5ced
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Widersten, Mikael

Search in DiVA

By author/editor
Widersten, Mikael
By organisation
Department of Chemistry - BMC
In the same journal
Current opinion in chemical biology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 538 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 853 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf