Probing Physical Properties of a DNA- Protein Complex Using Nanofluidic Channels
2014 (English)In: Small, ISSN 1613-6810, Vol. 10, no 5, 884-887 p.Article in journal (Refereed) Published
A method to investigate physical properties of a DNA-protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double-stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces.
Place, publisher, year, edition, pages
2014. Vol. 10, no 5, 884-887 p.
DNA, fluorescence, lipid passivation, nanofluidics, proteins
IdentifiersURN: urn:nbn:se:uu:diva-222757DOI: 10.1002/smll.201302028ISI: 000332343400007OAI: oai:DiVA.org:uu-222757DiVA: diva2:712179