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The production of the eosinophil proteins ECP and EPX/EDN are regulated in a reciprocal manner
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Respiratory Medicine and Allergology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
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2014 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 122, no 4, 283-291 p.Article in journal (Refereed) Published
Abstract [en]

Previous studies showed that the biological activity and the eosinophil content of eosinophil cationic protein (ECP, RNase 3) are determined by single-nucleotide polymorphisms (SNPs) in the ECP (RNase3) gene. In this study, we report the prevalence of a common SNP in the eosinophil protein x/eosinophil-derived neurotoxin (EPX/EDN, RNase2) and the association with the cellular contents of EPX/EDN and ECP. The genes were sequenced and the EPX/EDN405(G>C) rs2013109 SNPs were also determined by TaqMan 5nuclease allelic discrimination assay. ECP and EPX/EDN in purified eosinophils or in whole blood extracts were analysed by sensitive immunoassays. The study included 379 non-allergic and allergic subjects. The genotype prevalence of the EPX/EDN405(G>C) polymorphism was GG 59%, GC 36% and CC 6%. The cellular contents of ECP and EPX/EDN were related in a reciprocal fashion with the sums of the protein contents being constant. The contents were associated with the ECP562(G>C) rs2233860 and EPX/EDN405(G>C) gene polymorphisms. The cellular content of eosinophil peroxidase (EPO) was not associated with the ECP and EPX/EDN genotypes. The prevalence of the EPX/EDN405(G>C) genotypes and the contents of the proteins were similar in non-allergic and allergic subjects. The production and storage of the two ancestral proteins, ECP and EPX/EDN likely share common regulatory mechanisms, which result in opposing productions of the two proteins.

Place, publisher, year, edition, pages
2014. Vol. 122, no 4, 283-291 p.
Keyword [en]
Allergy, eosinophils, ECP, eosinophil cationic protein, EPX, EDN, eosinophil protein x, eosinophil-derived neurotoxin, genetics
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-222922DOI: 10.1111/apm.12142ISI: 000332206700003OAI: oai:DiVA.org:uu-222922DiVA: diva2:713052
Available from: 2014-04-17 Created: 2014-04-15 Last updated: 2017-12-05Bibliographically approved

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Jönsson, Ulla-BrittBlom, KristinStålenheim, GunnemarDouhan Håkansson, LenaVenge, Per

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Jönsson, Ulla-BrittBlom, KristinStålenheim, GunnemarDouhan Håkansson, LenaVenge, Per
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