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Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
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2014 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 5, p. 3309-Article in journal (Refereed) Published
Abstract [en]

Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-mu m-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

Place, publisher, year, edition, pages
2014. Vol. 5, p. 3309-
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Natural Sciences
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URN: urn:nbn:se:uu:diva-223577DOI: 10.1038/ncomms4309ISI: 000332668300002OAI: oai:DiVA.org:uu-223577DiVA, id: diva2:713976
Available from: 2014-04-24 Created: 2014-04-22 Last updated: 2017-12-05Bibliographically approved

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Seibert, Marvin M.

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