Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
2014 (English)In: Nature Communications, ISSN 2041-1723, Vol. 5, 3309- p.Article in journal (Refereed) Published
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-mu m-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
Place, publisher, year, edition, pages
2014. Vol. 5, 3309- p.
IdentifiersURN: urn:nbn:se:uu:diva-223577DOI: 10.1038/ncomms4309ISI: 000332668300002OAI: oai:DiVA.org:uu-223577DiVA: diva2:713976