The Drosophila ETV5 homologue Ets96B modulates feeding behavior
(English)Manuscript (preprint) (Other academic)
Recent genome-wide association studies (GWAS) have linked the PEA3-family member ets variant 5 (ETV5) to BMI and obesity, yet how this gene regulates metabolic homeostasis is still not clear. The PEA3-family was found to be remarkably conserved and present in species from Drosophila melanogaster to humans. The Drosophila PEA3-family homologue Ets96B is expressed in both the larval and adult central nervous system and, similar to mammalian ETV5, is highly expressed in the testis. In the current study we demonstrate that the obesity-linked homologue Ets96B regulates feeding behavior, as well as lipid storage in adult flies. Furthermore, we demonstrate this is a developmental phenotype. Of notable interest, when Ets96B was knocked down in the entire CNS or specifically in Ets96B expressing cells from embryogenesis, feeding behavior and lipid storage phenotypes were observed; yet when Ets96B was specifically knocked down in the adult CNS there was no effect on feeding, while an opposite effect on lipid storage was revealed. From these data we speculate that loss of Ets96B may disrupt CNS development, leading to metabolic homeostatic phenotypes.
Research subject Medical Science
IdentifiersURN: urn:nbn:se:uu:diva-223808OAI: oai:DiVA.org:uu-223808DiVA: diva2:714277