uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Arabidopsis replacement histone variant H3.3 occupies promoters of regulated genes
Show others and affiliations
2014 (English)In: Genome Biology, ISSN 1465-6906, E-ISSN 1474-760X, Vol. 15, no 4, R62- p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Histone variants establish structural and functional diversity of chromatin by affecting nucleosome stability and histone-protein interactions. H3.3 is an H3 histone variant that is incorporated into chromatin outside of S-phase in various eukaryotes. In animals, H3.3 is associated with active transcription and possibly maintenance of transcriptional memory. Plant H3 variants, which evolved independently of their animal counterparts, are much less well understood.

RESULTS: We profile the H3.3 distribution in Arabidopsis at mono-nucleosomal resolution using native chromatin immunoprecipitation. This results in the precise mapping of H3.3-containing nucleosomes, which are not only enriched in gene bodies as previously reported, but also at a subset of promoter regions and downstream of the 3[prime] ends of active genes. While H3.3 presence within transcribed regions is strongly associated with transcriptional activity, H3.3 at promoters is often independent of transcription. In particular, promoters with GA motifs carry H3.3 regardless of the gene expression levels. H3.3 on promoters of inactive genes is associated with H3K27me3 at gene bodies. In addition, H3.3-enriched plant promoters often contain RNA Pol II considerably upstream of the transcriptional start site. H3.3 and RNA Pol II are found on active as well as on inactive promoters and are enriched at strongly regulated genes.

CONCLUSIONS: In animals and plants, H3.3 organizes chromatin in transcribed regions and in promoters. The results suggest a function of H3.3 in transcriptional regulation and support a model that a single ancestral H3 evolved into H3 variants with similar sub-functionalization patterns in plants and animals.

Place, publisher, year, edition, pages
2014. Vol. 15, no 4, R62- p.
National Category
URN: urn:nbn:se:uu:diva-223952DOI: 10.1186/gb-2014-15-4-r62PubMedID: 24708891OAI: oai:DiVA.org:uu-223952DiVA: diva2:714532
Available from: 2014-04-28 Created: 2014-04-28 Last updated: 2014-09-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Siretskiy, Alexey
By organisation
Science for Life Laboratory, SciLifeLab
In the same journal
Genome Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 196 hits
ReferencesLink to record
Permanent link

Direct link