Induction kinetics of the lac operon: Studied by single molecule methods
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
The repression of the E. coli lac operon seems to be more efficient than the current theoretical model allows for. Specifically, it is more quiet than expected during the replication of the chromosome. I have induced cells during short periods and counted the number of protein products from the operon to determine if there is a delay in activation of transcription that could account for the discrepancy. The results are compatible with a delay of 10-20 s, but the delay could not be conclusively proven. Furthermore, it has been investigated if the mechanism behind the delay might be differential localization of the lac operon with and without induction. It is shown that the lac operon is more often located in the periphery of the cell and in the internucleoid region when induced. These might be regions where genes are higher expressed, giving a delay in expression after de-repression before the gene is transported there.
Place, publisher, year, edition, pages
2014. , 33 p.
Lac operon, repressor, transcription, single molecule fluorescence
Biophysics Cell Biology
IdentifiersURN: urn:nbn:se:uu:diva-224220OAI: oai:DiVA.org:uu-224220DiVA: diva2:716350
Molecular Biotechnology Engineering Programme
Elf, Johan, Professor