Localization of sirtuins in the mouse inner ear
2014 (English)In: Acta Oto-Laryngologica, ISSN 0001-6489, Vol. 134, no 4, 331-338 p.Article in journal (Refereed) Published
Conclusion: It is suggested that SIRT1 and 3, and probably SIRT4 and 5, play an important role in the neuroprotection of the inner ear. SIRT2 may be related to neuroprotection and myelin sheath formation, while SIRT6 seems to have a significant role in maintaining the energy balance by metabolic regulation. Objective: To analyze the expression of sirtuins (SIRT1-7) in the normal mouse inner ear. Methods: CBA/J mice were used for this study. The localization of SIRT1-7 in the inner ear, i.e. cochlea, vestibular end organs, and endolymphatic sac, was investigated using real-time PCR and immunohistochemistry. Results: We found high levels of mRNA of all seven sirtuins in the inner ear. In the immunohistochemical study, SIRT1-7 were abundant in many inner ear structures, i.e. stria vascularis, inner and outer hair cells, spiral ganglion cells, vestibular sensory and ganglion cells, vestibular dark and transitional cells, and the endolymphatic sac.
Place, publisher, year, edition, pages
2014. Vol. 134, no 4, 331-338 p.
Aquaporins, vasopressin receptor, immunohistochemistry
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-223860DOI: 10.3109/00016489.2013.861928ISI: 000333037200001OAI: oai:DiVA.org:uu-223860DiVA: diva2:716410