Detection of prognostic biomarkers with solid-phase proximity ligation assay in patients with colorectal cancer
2016 (English)In: Translational Oncology, ISSN 1944-7124, E-ISSN 1936-5233, Vol. 9, no 3, 251-255 p.Article in journal (Refereed) Published
Background: In the search for prognostic biomarkers a significant amount of precious biobanked blood samples is needed if conventional analyses are used. Solid-phase proximity ligation assay (SP-PLA) is an analytic method with the ability to analyse many proteins at the same time in small amounts of plasma. The aim of this study was to explore the potential use of SP-PLA in patients with colorectal cancer (CRC).
Material and methods: Plasma from patients with stage I-IV CRC, with (n=31) and without (n=29) disease dissemination at diagnosis or later, was analysed with SP-PLA using 35 antibodies targeting an equal number of proteins in 5 ml plasma. Carcinoembryonic antigen (CEA), analysed earlier on this cohort, was used as a reference.
Results: A total of 21 of the 35 proteins were detectable with SP-PLA. Patients in stage II-III with disseminated disease had lower plasma concentrations of HCC-4 (p=0.025). Low plasma levels of TIMP-1 were seen in patients with disseminated disease stage II (p=0.003). The level of CEA was higher in patients with disease dissemination compared to those without (p=0.007).
Conclusion: SP-PLA has the ability to analyse many tumour markers simultaneously in a small amount of blood. However, none of the markers selected for the present SP-PLA analyses gave better prognostic information compared with CEA.
Place, publisher, year, edition, pages
2016. Vol. 9, no 3, 251-255 p.
colorectal cancer, biomarkers, SP-PLA, recurrence, prognosis
Cancer and Oncology
IdentifiersURN: urn:nbn:se:uu:diva-224696DOI: 10.1016/j.tranon.2016.04.001ISI: 000378028300014PubMedID: 27267845OAI: oai:DiVA.org:uu-224696DiVA: diva2:717856
FunderEU, FP7, Seventh Framework Programme, 294409; 259796