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Turnover of DNA-P and phospholipid-P in lake sediments
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics.
2014 (English)In: Biogeochemistry, ISSN 0168-2563, E-ISSN 1573-515X, Vol. 119, no 1-3, 361-370 p.Article in journal (Refereed) Published
Abstract [en]

Identifying and quantifying the forms of phosphorus (P) in lake sediments is a prerequisite for understanding lake trophic status and possible exports of P downstream. Organic P is one of the most important P forms found in the sediment, where orthophosphate diesters, including DNA and phospholipids, represent a degradable P pool that can support primary production and eutrophication. In this study, sediment cores from the eutrophic Lake Erken and the oligotrophic Lake Ånnsjön, both in steady state regarding long-term P input revealed trends in the degradation of DNA-P and PL-P with sediment depth. Comparisons were performed based on the differentiation of essentially permanent or recalcitrant P and temporary, potentially mobile P for the respective fractions. The temporary P pool was defined as the part of the total P pool calculated for values higher than the level at which the measured P concentration converged to a constant value and the recalcitrant pool was defined as the difference between the total and the temporary. The temporary diester-P pool comprised over 20 % of the total temporary P in Lake Erken and around 4 % in Lake Ånnsjön. The decrease in P concentrations with depth was more rapid for DNA-P compared to PL-P in both lakes, suggesting that DNA-P has a more prominent role in internal loading. The study shows that P mobilization potential can be different for different P fractions, which is important when assessing their contribution to internal loading of P within an aquatic system.

Place, publisher, year, edition, pages
2014. Vol. 119, no 1-3, 361-370 p.
Keyword [en]
Phosphorus turnover; Sediment; DNA; Phospholipids; Erken; Ånnsjön
National Category
Earth and Related Environmental Sciences Chemical Sciences
URN: urn:nbn:se:uu:diva-224699DOI: 10.1007/s10533-014-9972-3ISI: 000336028400023OAI: oai:DiVA.org:uu-224699DiVA: diva2:717999
Available from: 2014-05-19 Created: 2014-05-19 Last updated: 2014-09-08Bibliographically approved
In thesis
1. Organic phosphorus speciation in environmental samples: Method development and applications
Open this publication in new window or tab >>Organic phosphorus speciation in environmental samples: Method development and applications
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis investigates the development of new methodology for the identification and quantification of organic phosphorus compounds in environmental samples.

Phosphorus is a vital element for primary production and one of the factors contributing to eutrophication. Eutrophication of aquatic systems leads to algal blooms, changes in ecological balance and deteriorating water quality. Difficulties in studying organic phosphorus stem from the fact that organic phosphorus is present in the environment in a variety of forms and each form may have different degradation and turnover time, having very different effects on eutrophication.

New methods for the quantification of phosphorus derived from three groups of organic phosphorus compounds were developed. For the determination of phosphorus derived from DNA and phospholipids selective extraction was combined with digestion and colorimetric determination of the extracted phosphate. For quantification of inositol phosphates high performance liquid chromatography was coupled with tandem mass spectrometry using electrospray ionization.  

The methods were applied to studying the distribution of these compounds in a small catchment and in the case of DNA-P and phospholipid-P, the degradation of the fractions in lake sediments. The studies showed that phosphorus bound to DNA, phospholipids and inositol phosphates constitute a sizeable part of the total phosphorus in different environmental samples. The phospholipid-P fraction was the smallest one, accounting for, on average, only a few percent of the total phosphorus in the sample. Inositol phosphates were most prevalent in the soils, with inositol hexakisphosphate accounting for over 10% of the total phosphorus content. The highest content of DNA-P was found in sediments and it was shown that DNA-P degrades more rapidly than phospholipid-P and therefore plays a more critical role in internal loading.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2014. 57 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1156
Organic phosphorus, DNA, phospholipids, inositol phosphates, sediment, soil, extraction, liquid chromatography (LC), mass spectrometry (MS)
National Category
Analytical Chemistry
Research subject
Chemistry with specialization in Analytical Chemistry
urn:nbn:se:uu:diva-228734 (URN)978-91-554-8981-6 (ISBN)
Public defence
2014-09-08, B41, BMC, Husargatan 3, Uppsala, 09:15 (English)
Available from: 2014-08-18 Created: 2014-07-21 Last updated: 2014-09-08

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Publisher's full texthttp://dx.doi.org/10.1007/s10533-014-9972-3

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Paraskova, Julia V.Sjöberg, Per J.R.Rydin, Emil
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