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X-ray microanalysis in the scanning electron microscope
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular and Morphological Pathology.
2014 (English)In: Electron Microscopy: Methods and Protocols / [ed] John Kuo, New York: Humana Press, 2014, 3rd, Vol. 1117, 639-661 p.Chapter in book (Refereed)
Abstract [en]

X-ray microanalysis conducted using the scanning electron microscope is a technique that allows the determination of chemical elements in bulk or semi-thick specimens. The lowest concentration of an element that can be detected is in the order of a few mmol/kg or a few hundred parts per million, and the smallest amount is in the order of 10(-18) g. The spatial resolution of the analysis depends on the thickness of the specimen. For biological specimen analysis, care must be taken to prevent displacement/loss of the element of interest (usually ions). Protocols are presented for the processing of frozen-hydrated and freeze-dried specimens, as well as for the analysis of small volumes of fluid, cell cultures, and other specimens. Aspects of qualitative and quantitative analysis are covered, including limitations of the technique.

Place, publisher, year, edition, pages
New York: Humana Press, 2014, 3rd. Vol. 1117, 639-661 p.
Series
Methods in Molecular Biology: Methods and Protocols, ISSN 1064-3745 ; 1117
National Category
Clinical Laboratory Medicine Basic Medicine
Research subject
Pathology
Identifiers
URN: urn:nbn:se:uu:diva-224757DOI: 10.1007/978-1-62703-776-1_28ISI: 000341167200029PubMedID: 24357383ISBN: 978-1-62703-775-4 (print)ISBN: 978-1-62703-776-1 (print)OAI: oai:DiVA.org:uu-224757DiVA: diva2:718114
Available from: 2014-05-19 Created: 2014-05-19 Last updated: 2014-10-07Bibliographically approved

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