uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Dark-adapted spinach thylakoid protein heterogeneity offers insights into the Photosystem II repair cycle
Show others and affiliations
2014 (English)In: Biochimica et Biophysica Acta - Bioenergetics, ISSN 0005-2728, E-ISSN 1879-2650, Vol. 1837, no 9, 1463-1471 p.Article in journal (Refereed) Published
Abstract [en]

In higher plants, thylakoid membrane protein complexes show lateral heterogeneity in their distribution: photosystem (PS) II complexes are mostly located in grana stacks, whereas PSI and adenosine triphosphate (ATP) synthase are mostly found in the stroma-exposed thylakoids. However, recent research has revealed strong dynamics in distribution of photosystems and their light harvesting antenna along the thylakoid membrane. Here, the dark-adapted spinach (Spinacia oleracea L.) thylakoid network was mechanically fragmented and the composition of distinct PSII-related proteins in various thylakoid subdomains was analyzed in order to get more insights into the composition and localization of various PSII subcomplexes and auxiliary proteins during the PSII repair cycle. Most of the PSII subunits followed rather equal distribution with roughly 70% of the proteins located collectively in the grana thylakoids and grana margins; however, the low molecular mass subunits PsbW and PsbX as well as the PsbS proteins were found to be more exclusively located in grana thylakoids. The auxiliary proteins assisting in repair cycle of PSII were mostly located in stroma-exposed thylakoids, with the exception of THYLAKOID LUMEN PROTEIN OF 18.3 (TLP18.3), which was more evenly distributed between the grana and stroma thylakoids. The TL29 protein was present exclusively in grana thylakoids. Intriguingly, PROTON GRADIENT REGULATION5 (PGR5) was found to be distributed quite evenly between grana and stroma thylakoids, whereas PGR5-LIKE PHOTOSYNTHETIC PHENOTYPE1 (PGRL1) was highly enriched in the stroma thylakoids and practically missing from the grana cores.

This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy.

Place, publisher, year, edition, pages
2014. Vol. 1837, no 9, 1463-1471 p.
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-224806DOI: 10.1016/j.bbabio.2013.11.014ISI: 000341474300011PubMedID: 24296034OAI: oai:DiVA.org:uu-224806DiVA: diva2:718252
Available from: 2014-05-20 Created: 2014-05-20 Last updated: 2014-10-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Styring, StenbjörnMamedov, Fikret
By organisation
Molecular Biomimetics
In the same journal
Biochimica et Biophysica Acta - Bioenergetics
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 476 hits
ReferencesLink to record
Permanent link

Direct link