Induction of Gliotoxin Secretion in Aspergillus fumigatus by Bacteria-Associated Molecules
2014 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 9, no 4, e93685- p.Article in journal (Refereed) Published
Aspergillus fumigatus is the most common causative agent of mold diseases in humans, giving rise to life-threatening infections in immunocompromised individuals. One of its secreted metabolites is gliotoxin, a toxic antimicrobial agent. The aim of this study was to determine whether the presence of pathogen-associated molecular patterns in broth cultures of A. fumigatus could induce gliotoxin production. Gliotoxin levels were analyzed by ultra-performance liquid chromatography and mass spectrometry. The presence of a bacteria-derived lipopolysaccharide, peptidoglycan, or lipoteichoic acid in the growth media at a concentration of 5 mu g/ml increased the gliotoxin concentration in the media by 37%, 65%, and 35%, respectively. The findings reveal a correlation between the concentrations of pathogen-associated molecular patterns and gliotoxin secretion. This shows that there is a yet uncharacterized detection system for such compounds within fungi. Inducing secondary metabolite production by such means in fungi is potentially relevant for drug discovery research. Our results also give a possible explanation for the increased virulence of A. fumigatus during bacterial co-infection, one that is important for the transition from colonization to invasiveness in this pulmonary disease.
Place, publisher, year, edition, pages
2014. Vol. 9, no 4, e93685- p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-224740DOI: 10.1371/journal.pone.0093685ISI: 000334107500056OAI: oai:DiVA.org:uu-224740DiVA: diva2:718406