uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Stem cell marker-positive stellate cells and mast cells are reduced in benign-appearing bladder tissue in patients with urothelial carcinoma
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Urology.
Show others and affiliations
2014 (English)In: Virchows Archiv, ISSN 0945-6317, E-ISSN 1432-2307, Vol. 464, no 4, 473-488 p.Article in journal (Refereed) Published
Abstract [en]

Survival after invasive bladder cancer has improved less than that of other common non-skin cancers. In many types of malignancy, treatment failure has been attributed to therapy-resistant stem-like cancer cells. Our aim was therefore to determine identities of stem cell marker-positive cells in bladder cancer tissue and to investigate possible associations between these cells and different forms of bladder neoplasia. We investigated tissue from 52 patients with bladder neoplasia and 18 patients with benign bladder conditions, from a cohort that had been previously described with regard to diagnosis and outcome. The samples were analysed immunohistologically for the stem cell markers aldehyde dehydrogenase 1 A1 (ALDH1) and CD44, and markers of cell differentiation. The majority of stem cell marker-positive cells were located in connective tissue, and a smaller fraction in epithelial tissue. Stem cell marker-positive cells exhibiting possible stem cell characteristics included cells in deeper locations of benign and malignant epithelium, and sub-endothelial cells in patients with or without neoplasia. Stem cell marker-positive cells with non-stem cell character included stellate cells, mast cells, endothelial cells, foamy histiocytes, and neurons. Significantly, ALDH1+ stellate cells and ALDH1+ mast cells were reduced in number in stroma of benign-appearing mucosa of bladder cancer patients. The stem cell markers ALDH1 and CD44 label several types of differentiated cells in bladder tissue. ALDH1+ stellate cells and mast cells appear to be reduced in stroma of normal-appearing mucosa of bladder cancer patients, and may be part of a "field effect" in cancer-near areas.

Place, publisher, year, edition, pages
2014. Vol. 464, no 4, 473-488 p.
Keyword [en]
Bladder cancer, Tumour microenvironment, Stem cells, Stellate cells, Mast cells, Aldehyde dehydrogenase, CD44
National Category
Urology and Nephrology
URN: urn:nbn:se:uu:diva-224916DOI: 10.1007/s00428-014-1561-2ISI: 000334174000010OAI: oai:DiVA.org:uu-224916DiVA: diva2:720187
Available from: 2014-05-28 Created: 2014-05-23 Last updated: 2014-05-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Busch, Christer
By organisation
In the same journal
Virchows Archiv
Urology and Nephrology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 266 hits
ReferencesLink to record
Permanent link

Direct link