Specific spatial distribution of caspase-3 in normal lenses
2014 (English)In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 93, no 3, 289-292 p.Article in journal (Refereed) Published
To determine the distribution of active caspase-3 in rat eye lens epithelium.
In total, 120 sagittal sections from forty rats were assessed for active caspase-3 labelling using immunohistochemistry. Lens epithelial cells were counted, and the fraction of active caspase-3 labelled cells and their relative positions were identified in each section.
Active caspase-3 is present in normal lens epithelium. The active caspase-3 expression was higher in the anterior pole of the lens. Probability of radial spatial distribution of labelling was fitted with a logistic model. The increase rate and the inflection point were estimated as CI (0.95) to 23 ± 3 cells and 114 ± 3 cells, respectively.
The gradually decreasing active caspase-3 labelling from the anterior pole to the periphery suggests that active caspase-3 may be involved in normal protein turnover caused by, for example, incident light.
Place, publisher, year, edition, pages
2014. Vol. 93, no 3, 289-292 p.
Research subject Ophtalmology
IdentifiersURN: urn:nbn:se:uu:diva-226542DOI: 10.1111/aos.12501ISI: 000353053000031OAI: oai:DiVA.org:uu-226542DiVA: diva2:726383