Vasostatin-1 antigenic epitope mapping for induction of cellular and humoral immune responses to chromogranin A autoantigen in NOD mice
2014 (English)In: European Journal of Immunology, ISSN 0014-2980, E-ISSN 1521-4141, Vol. 44, no 4, 1170-1180 p.Article in journal (Refereed) Published
The chromogranin A (ChgA) 29-42 sequence is the antigenic epitope for the BDC2.5 CD4(+) T-cell receptor in NOD mice (H-2(g7)). We have now characterized the binding register of the ChgA 29-42 peptide for the I-A(g7) molecule. Truncation of the peptide demonstrated that the KCVLEVISD sequence 34-42 is the binding register and extension of this sequence by flanking residues increased its binding affinity and antigenic capacity. We employed anti-ChgA peptide antibodies generated against different fragments of ChgA for immunostaining of pancreatic islet sections from NOD mice. A strong immuno-staining pattern was observed for the ChgA 17-38 peptide antibodies that overlap with the ChgA 29-42 sequence. Moreover, sera from diabetic NOD mice showed elevated titers of autoantibodies to the ChgA 29-42 peptide. These findings indicate that peptides from the N-terminal region of ChgA are able to induce cellular and humoral immune responses in NOD mice.
Place, publisher, year, edition, pages
2014. Vol. 44, no 4, 1170-1180 p.
BDC2, 5 T cells, Chromogranin A, I-A(g7) peptide binding motif, Type 1 diabetes
Immunology in the medical area
IdentifiersURN: urn:nbn:se:uu:diva-225078DOI: 10.1002/eji.201343986ISI: 000334174400028OAI: oai:DiVA.org:uu-225078DiVA: diva2:727209