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Stability of serum analytes depending on pre-centrifugation time and platelet count in three types of sample collecting tubes
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
2014 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

The pre-analytical phase of a blood sample is known to be very important due to the need of correct preparation and handling of the sample. If these are not executed correctly, the test results can differ from the true value. The aim of this study was to evaluate the stability of 8 serum analytes depending on pre-centrifugation time and to compare platelet count in three types of sample collecting tubes. The study was performed in a hospital laboratory with routine instruments, which are normally used for patient samples. Blood for the serum analyses was drawn from 14 people and for the platelet count from 15 people. The included analytes were alanine aminotransferase, aspartate transaminase, phosphate, calcium, potassium, lactate dehydrogenase, magnesium and sodium. The results showed that aspartate transaminase, calcium, potassium and sodium were stable for up to 4 hours in non-centrifuged tubes. Alanine aminotransferase, phosphate, lactate dehydrogenase and magnesium were less stable but the variations were not clinically relevant. Platelet count was the same in blood anticoagulated with ethylenediaminetetraacetic acid (EDTA) and sodium citrate of two concentrations. The conclusion of the study was that none of the serum analytes showed a clinically relevant difference due to a delayed pre-centrifugation time. Platelet count is not affected by the choice of sample collecting tubes.

Place, publisher, year, edition, pages
Keyword [en]
Pre-analytical factors, Gel tubes, Pseudotrombocytopenia, EDTA, Sodium citrate
National Category
Clinical Laboratory Medicine
URN: urn:nbn:se:uu:diva-226938OAI: oai:DiVA.org:uu-226938DiVA: diva2:727793
Educational program
Biomedical Laboratory Science Programme
Available from: 2014-06-24 Created: 2014-06-23 Last updated: 2014-06-24Bibliographically approved

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