In vitro binding of [H-3]PIB to human amyloid deposits of different types
2014 (English)In: Amyloid: Journal of Protein Folding Disorders, ISSN 1350-6129, Vol. 21, no 1, 21-27 p.Article in journal (Refereed) Published
Systemic amyloidosis is caused by extracellular deposition of insoluble fibrillar proteins arranged in beta-pleated sheets. [C-11] PIB has been used in PET studies to assess A beta deposition in brain of patients with Alzheimer's disease (AD). The possibility to visualize other types of amyloid deposits with [C-11] PIB would be of potential clinical importance in early diagnosis and for following therapeutic effects. In the present study, we evaluated in vitro binding of [3 H] PIB to tissues containing transthyretin (ATTR), immunoglobulin light-chain (AL), amyloid protein A (AA) and Ab amyloid. We found significantly higher binding of [H-3] PIB in tissue from systemic amyloidoses than in control tissue, i.e. 4.7 times higher (p<0.05). [H-3] PIB showed the highest affinity to cortex of AD brain (IC50 = 3.84 nM), while IC50 values were much higher for ATTR, AA and AL type of amyloidosis and large variations in affinity were observed even within tissues having the same type of amyloidosis. Extraction with guanidine-HCl, which disrupts the beta-sheet structure, decreased the protein levels and, concomitantly, the binding of [H-3] PIB in all four types of amyloidoses.
Place, publisher, year, edition, pages
2014. Vol. 21, no 1, 21-27 p.
A beta, AL, Alzheimer's disease, ATTR amyloidosis, light-chain amyloidosis, senile systemic amyloidosis, systemic amyloidosis, transthyretin
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-227132DOI: 10.3109/13506129.2013.860895ISI: 000335764200004OAI: oai:DiVA.org:uu-227132DiVA: diva2:728743