uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Ontogenetic development and spatial distribution of the ileal apical sodium-dependent bile acid transporter and the ileal lipid-binding protein in apoE knockout and C57BL/6 mice
AstraZeneca R&D Mo¨lndal, Discovery, Mo¨lndal, Sweden.
Show others and affiliations
2002 (English)In: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, Vol. 37, no 9, 1089-1096 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Although apoE-/- mice are characterized by hypercholesterolemia, the bile acid enterohepatic circulation, which plays a crucial role in cholesterol homeostasis, has not been examined in these mice. The differences between apoE-/- and C57BL/6 mice in expression of the ileal ASBT and ILBP and in intestinal bile acid absorption were studied.

METHODS: The intestinal tissues of the fetal, neonatal and post-weaning mice were processed for immunohistochemistry. Body retention and fecal excretion of 75SeHCAT were measured. The bile acid pool size and its composition were analysed by HPLC.

RESULTS: In apoE-/- and C57BL/6 mice, the bile acid pool size was 75 +/- 13 and 78 +/- 13 micromol/ 100 g body weight, respectively, while the ratio of cholic acid/beta-muricholic acid was 1.8 +/- 0.3 and 1.4 +/- 0.3 (P < 0.05), respectively. The daily body retention of 75SeHCAT was 48% = 1.8% in C57 black mice and 58.4% +/- 2.7% in apoE-/- mice (P < 0.05). In both mouse strains, ASBT expression in the small intestine was found in the near-term fetal and post-weaning mice, while ILBP expression was found in all postnatal mice. In the post-weaning mice, ILBP expression was limited to the distal 25%-30% of the small intestine, while ASBT expression was limited to the distal 18%.

CONCLUSIONS: The bile acid enterohepatic circulation in apoE-/- mice probably does not differ greatly from that in C57BL/6 mice.

Place, publisher, year, edition, pages
2002. Vol. 37, no 9, 1089-1096 p.
National Category
Chemical Sciences Medical and Health Sciences
URN: urn:nbn:se:uu:diva-227545DOI: 10.1080/003655202320378301PubMedID: 12374235OAI: oai:DiVA.org:uu-227545DiVA: diva2:730227
Available from: 2014-06-27 Created: 2014-06-27 Last updated: 2014-09-17Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Andersson, Ingela
In the same journal
Scandinavian Journal of Gastroenterology
Chemical SciencesMedical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 411 hits
ReferencesLink to record
Permanent link

Direct link