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Organization of Ribosomes and Nucleoids in Escherichia coli Cells during Growth and in Quiescence
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
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2014 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 289, no 16, 11342-11352 p.Article in journal (Refereed) Published
Abstract [en]

Background: We studied ribosome and nucleoid distribution in Escherichia coli under growth and quiescence. Results: Spatially segregated ribosomes and nucleoids show drastically altered distribution in stationary phase or when treated with drugs affecting translation, transcription, nucleoid-topology, or cytoskeleton. Ribosome inheritance in daughter cells is frequently unequal. Conclusion: Cellular growth processes modulate ribosome and nucleoid distribution. Significance: This provides insight into subcellular organization of molecular machines. We have examined the distribution of ribosomes and nucleoids in live Escherichia coli cells under conditions of growth, division, and in quiescence. In exponentially growing cells translating ribosomes are interspersed among and around the nucleoid lobes, appearing as alternative bands under a fluorescence microscope. In contrast, inactive ribosomes either in stationary phase or after treatment with translation inhibitors such as chloramphenicol, tetracycline, and streptomycin gather predominantly at the cell poles and boundaries with concomitant compaction of the nucleoid. However, under all conditions, spatial segregation of the ribosomes and the nucleoids is well maintained. In dividing cells, ribosomes accumulate on both sides of the FtsZ ring at the mid cell. However, the distribution of the ribosomes among the new daughter cells is often unequal. Both the shape of the nucleoid and the pattern of ribosome distribution are also modified when the cells are exposed to rifampicin (transcription inhibitor), nalidixic acid (gyrase inhibitor), or A22 (MreB-cytoskeleton disruptor). Thus we conclude that the intracellular organization of the ribosomes and the nucleoids in bacteria are dynamic and critically dependent on cellular growth processes (replication, transcription, and translation) as well as on the integrity of the MreB cytoskeleton.

Place, publisher, year, edition, pages
2014. Vol. 289, no 16, 11342-11352 p.
Keyword [en]
Antibiotics, Cell Division, Cytoskeleton, DNA Replication, Ribosomes, Subcellular Organelles, Transcription, Translation, FtsZ Ring, Nucleoid
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-227282DOI: 10.1074/jbc.M114.557348ISI: 000334638500036OAI: oai:DiVA.org:uu-227282DiVA: diva2:730821
Funder
Swedish Research Council, 2010-2619, 2011-6088, 2008-6593Carl Tryggers foundation , 09:341, 10:330Knut and Alice Wallenberg Foundation, 2011.0081
Available from: 2014-06-30 Created: 2014-06-24 Last updated: 2017-12-05Bibliographically approved

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Peisker, KristinGe, XueliangDasgupta, SantanuSanyal, Suparna

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