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Transplantation of Co-aggregates of Sertoli Cells and Islet Cells Into Liver Without Immunosuppression
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
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2014 (English)In: Transplantation, ISSN 0041-1337, E-ISSN 1534-6080, Vol. 97, no 3, 287-293 p.Article in journal (Refereed) Published
Abstract [en]

Background Transplantation of islets of Langerhans (islets) was used to treat insulin-dependent diabetes mellitus. However, islet grafts must be maintained by administration of immunosuppressive drugs, which can lead to complications in the long term. An approach that avoids immunosuppressive drug use is desirable. Methods Co-aggregates of Sertoli cells and islet cells from BALB/c mice that were prepared by the hanging drop method were transplanted into C57BL/6 mouse liver through the portal vein as in human clinical islet transplantation. Results The core part of the aggregates contained mainly Sertoli cells, and these cells were surrounded by islet cells. The co-aggregates retained the functions of both Sertoli and islet cells. When 800 co-aggregates were transplanted into seven C57BL/6 mice via the portal vein, six of seven recipient mice demonstrated quasi-normoglycemia for more than 100 days. Conclusions The hanging drop method is suitable for preparing aggregates of Sertoli and islet cells for transplantation. Notably, transplantation of these allogeneic co-aggregates into mice with chemically induced diabetes via the portal vein resulted in long-term graft survival without systemic immunosuppression.

Place, publisher, year, edition, pages
2014. Vol. 97, no 3, 287-293 p.
Keyword [en]
Islet of Langerhans, Intraportal transplantation, Sertoli cell, Co-aggregate, Allotransplantation
National Category
Immunology Surgery
URN: urn:nbn:se:uu:diva-227944DOI: 10.1097/01.TP.0000438198.76531.03ISI: 000335838500013OAI: oai:DiVA.org:uu-227944DiVA: diva2:733003
Available from: 2014-07-07 Created: 2014-07-02 Last updated: 2014-07-07Bibliographically approved

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Teramura, Yuji
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Clinical Immunology
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