uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Mass spectrometry imaging of freeze-dried membrane phospholipids of dividing Tetrahymena pyriformis
Show others and affiliations
2013 (English)In: Surface and Interface Analysis, ISSN 0142-2421, E-ISSN 1096-9918, Vol. 45, no 1, 211-214 p.Article in journal (Refereed) Published
Abstract [en]

Time of Flight secondary ion mass spectrometry (TOF-SIMS) has been used to explore the distribution of phospholipids in the plasma membrane of Tetrahymena pyriformis during cell division. The dividing cells were freeze dried prior to analysis followed by line scan and region of interest analysis at various stages of cell division. The results showed no signs of phospholipid domain formation at the junction between the dividing cells. Instead the results showed that the sample preparation technique had a great impact on one of the examined phospholipids, namely phosphatidylcholine (PC). Phosphatidylcholine and 2-aminoethylphosphonolipid (2-AEP) have therefore been evaluated in Tetrahymena cells that have been subjected to different sample preparation techniques: freeze drying ex situ, freeze fracture, and freeze fracture with partial or total freeze drying in situ. The result suggests that freeze-drying ex situ causes the celia to collapse and cover the plasma membrane.

Place, publisher, year, edition, pages
2013. Vol. 45, no 1, 211-214 p.
National Category
Chemical Sciences
URN: urn:nbn:se:uu:diva-228336DOI: 10.1002/sia.5017PubMedID: 23264706OAI: oai:DiVA.org:uu-228336DiVA: diva2:733746
Available from: 2014-07-11 Created: 2014-07-11 Last updated: 2014-09-17Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Lanekoff, Ingela
In the same journal
Surface and Interface Analysis
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 386 hits
ReferencesLink to record
Permanent link

Direct link