Actin Filaments Attachment to the Plasma Membrane Cause the Formation of Ordered Lipid Domains in Live Cells
2014 (English)In: Biophysical Journal, ISSN 0006-3495, E-ISSN 1542-0086, Vol. 106, no 2, 706A-706A p.Article in journal, Meeting abstract (Other academic) Published
The aim of this study was to investigate the relationship between ordered plasma membrane nanodomains and actin filaments using di-4-ANEPPDHQ and laurdan together with the reagents that affect actin filament dynamics in live Jurkat and primary T cells. The degree of lipid packing can be quantified using polarity sensitive membrane dyes such as laurdan and di-4-ANEPPDHQ. These two dyes display a red shift in their emission peaks for membranes in ld phase relative to lo phase. Laurdan is uncharged and can easily flip between two leaflets of the plasma membrane and we demonstrate that it reports equally on the two leaflets of the plasma membrane.
Place, publisher, year, edition, pages
2014. Vol. 106, no 2, 706A-706A p.
IdentifiersURN: urn:nbn:se:uu:diva-228608DOI: 10.1016/j.bpj.2013.11.3904ISI: 000337000404033OAI: oai:DiVA.org:uu-228608DiVA: diva2:734550
58th Annual Meeting of the Biophysical-Society, FEB 15-19, 2014, San Francisco, CA