Probing Physical Properties of a DNA-Protein Complex Using Nanofluidic Channels
2014 (English)In: Biophysical Journal, ISSN 0006-3495, E-ISSN 1542-0086, Vol. 106, no 2, 428A-429A p.Article in journal, Meeting abstract (Other academic) Published
Nanofluidic channels have become an important tool to investigate single DNA molecules both from a fundamental polymer physics perspective as well as in e.g. optical mapping techniques. However, less effort has been made to study DNA-protein complexes. A main reason is that the extreme surface-to-volume ratio in the nanochannels causes most proteins to stick to the channel walls. We have recently overcome this problem by coating the channels with a lipid bilayer, thereby eliminating sticking.
Place, publisher, year, edition, pages
2014. Vol. 106, no 2, 428A-429A p.
IdentifiersURN: urn:nbn:se:uu:diva-228601DOI: 10.1016/j.bpj.2013.11.2415ISI: 000337000402404OAI: oai:DiVA.org:uu-228601DiVA: diva2:734563
58th Annual Meeting of the Biophysical-Society, FEB 15-19, 2014, San Francisco, CA