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Garbage in, garbage out: A critical evaluation of strategies used for validation of immunohistochemical biomarkers
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
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2014 (English)In: Molecular Oncology, ISSN 1574-7891, E-ISSN 1878-0261, Vol. 8, no 4, 783-798 p.Article, review/survey (Refereed) Published
Abstract [en]

The use of immunohistochemistry (IHC) in clinical cohorts is of paramount importance in determining the utility of a biomarker in clinical practice. A major bottleneck in translating a biomarker from bench-to-bedside is the lack of well characterized, specific antibodies suitable for IHC. Despite the widespread use of IHC as a biomarker validation tool, no universally accepted standardization guidelines have been developed to determine the applicability of particular antibodies for IHC prior to its use. In this review, we discuss the technical challenges faced by the use of immunohistochemical biomarkers and rigorously explore classical and emerging antibody validation technologies. Based on our review of these technologies, we provide strict criteria for the pragmatic validation of antibodies for use in immunohistochemical assays.

Place, publisher, year, edition, pages
2014. Vol. 8, no 4, 783-798 p.
Keyword [en]
Immunohistochemistry, Biomarker discovery, Antibody reliability, Antibody validation, Workflow
National Category
Cancer and Oncology Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-229616DOI: 10.1016/j.molonc.2014.03.008ISI: 000338405700002OAI: oai:DiVA.org:uu-229616DiVA: diva2:737332
Available from: 2014-08-12 Created: 2014-08-12 Last updated: 2017-12-05Bibliographically approved

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O'Hurley, GillianSjöstedt, EvelinaKampf, CarolinePontén, FredrikLindskog, Cecilia

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O'Hurley, GillianSjöstedt, EvelinaKampf, CarolinePontén, FredrikLindskog, Cecilia
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Science for Life Laboratory, SciLifeLabDepartment of Immunology, Genetics and Pathology
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Molecular Oncology
Cancer and OncologyCell and Molecular Biology

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