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The role of αB-crystallin in levodopa induced dyskinesia
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences, Toxicology.
2014 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

αB-crystallin (CRYAB), a small heat shock protein (sHSP) possibly of oligodendrocytic origin, is found to be up-regulated in brain tissues of untreated parkinsonian rats and in highly dyskinetic rats after treatment with levodopa (L-DOPA), whereas the levels are significantly lower in low dyskinetic animals. The aim of the project was to find out more about CRYAB´s role in untreated and highly dyskinetic animals. By using fluorescent immunohistochemistry (IHC) CRYAB was investigated to find out in what stage of the oligodendrocytic lineage the sHSP is expressed, also a cell culture method for CRYAB expression has been investigated. The results from experiments in healthy brain tissue, showed partly double positive cells for late premyelinating and myelinating oligodendrocyte markers CRYAB+/MAG+ and CRYAB+/RIP+. A method for creating CRYAB expressing cell culture with myelinating oligodendrocytes was examined and resulted in partly double positive cells for CRYAB+/MBP+ cells after addition of extra oligodendrocytes. The results indicate that CRYAB is expressed somewhere in between premyelinating and myelinating steps in the oligodendrocytic lineage. Also an oligodendrocytic cell culture expressing CRYAB have been developed for further investigations of CRYAB and its properties, but is in need of optimization for a better stability and standardization.

Place, publisher, year, edition, pages
Keyword [en]
CRYAB, Parkinson, AB-crystallin, levodopa, dyskinesia, LID, crystallin
National Category
Pharmacology and Toxicology
URN: urn:nbn:se:uu:diva-229149OAI: oai:DiVA.org:uu-229149DiVA: diva2:738056
Available from: 2014-08-28 Created: 2014-08-03 Last updated: 2014-08-28Bibliographically approved

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