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Developmentally regulated collagen/integrin interactions confer adhesive properties to early postnatal neural stem cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Cancer and Vascular Biology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Cancer and Vascular Biology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. (Staffan Johansson)
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2014 (English)In: Biochimica et Biophysica Acta - General Subjects, ISSN 0304-4165, E-ISSN 1872-8006, Vol. 1840, no 8, 2526-2532 p.Article in journal (Refereed) Published
Abstract [en]

Background:

It is becoming increasingly apparent that the extracellular matrix acts as an important regulator of the neural stem niche. Previously we found that neural stem and progenitor cells (NSPCs) derived from the early postnatal subventricular zone of mice adhere to a collagen/hyaluronan hydrogel, whereas NSPCs from the adult and embryonic brain do not.

Methods:

To examine the specific adhesive properties of young stem cells in more detail, NSPCs isolated from embryonic, postnatal day 6 (P6), and adult mouse brains were cultured on collagen I.

Results:

Early postnatal NSPCs formed paxillin-positive focal adhesions on collagen I, and these adhesions could be prevented by an antibody that blocked integrin beta 1. Furthermore, we found the corresponding integrin alpha subunits alpha 2 and alpha 11 levels to be highest at the postnatal stage. Gene ontology analysis of differentially expressed genes showed higher expression of transcripts involved in vasculature development and morphogenesis in P6 stem cells, compared to adult.

Conclusions:

The ability to interact with the extracellular matrix differs between postnatal and adult NSPCs.

General significance:

Our observations that the specific adhesive properties of early postnatal NSPCs, which are lost in the adult brain, can be ascribed to the integrin subunits expressed by the former furthering our understanding of the developing neurogenic niche. This article is part of a Special Issue entitled Matrix-mediated cell behaviour and properties.  

Place, publisher, year, edition, pages
2014. Vol. 1840, no 8, 2526-2532 p.
Keyword [en]
Cell adhesion, Integrin beta1, Integrin alpha, Neurovascular niche
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-230063DOI: 10.1016/j.bbagen.2014.01.021ISI: 000339035400015PubMedID: 24462579OAI: oai:DiVA.org:uu-230063DiVA: diva2:738924
Available from: 2014-08-19 Created: 2014-08-19 Last updated: 2017-12-05Bibliographically approved

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Bergström, TobiasJohansson, StaffanForsberg-Nilsson, Karin

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Cancer and Vascular BiologyScience for Life Laboratory, SciLifeLabDepartment of Medical Biochemistry and Microbiology
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