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Effekterna av frivlligt alkoholintag på CB1-receptordensiteten samt förekomst av lateralisering i råtthjärnan
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2014 (Swedish)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Background: Alcohol is a well-known intoxicant and has been used for thousands of years due to its reinforcing effects. High consumption and abuse of alcohol have however shown darker sides of the substance, since it is able to cause addiction and long-term changes in the CNS. The term for alcohol abuse and dependence are today renamed as ”alcohol use disorder” (AUD), which is characterized as uncontrolled and compulsive alcohol consumption. The biological mechanism for AUD remains unclear but genetics and variations in several biological systems seem to have an impact. The endocannabinoid system (ECS) regulates many different biological functions and is also of great importance in mechanisms behind reward and addiction. Furthermore does alcohol intake seem to increase if it simultaneously interacts with the ECS and the endocannabinoid receptor 1 (CB1R) and CB1R-antagonists seem to reduce alcohol intake. A few studies have also illuminated that the effects of alcohol and the function of ECS are lateralized and asymmetrically distributed between the hemispheres. The aim of this study was to investigate alcohol-induced effects on CB1R density following voluntary alcohol intake and analyze if the CB1R density are lateralized between left and right hemisphere. Methods: Wistar male rats were used in this study in order to investigate long-term changes in brain regarding CB1R density. The rats were introduced to ethanol by a two-bottle-free-choice paradigm where they had 24-hours intermittent access to 20 % ethanol three days a week for seven weeks. The rats were decapitated 10-11 days after the final ethanol intake and the brains were sliced into 12 μm sections at six different bregma levels. In order to analyze CB1R density in the rat brain, an autoradiographic method was used with the ligand [3H] SR171614A, and the sections were further analyzed densiometrically. Results: Ethanol-drinking rats showed altered CB1R density in four brain areas, relative to water-drinking rats. In addition, lateralisation in CB1R density was found regardless of ethanol intake. Conclusion: Voluntary intake of 20 % ethanol lead to alterations in several brain structures that are significant in mechanisms of reward, and in certain brain structures the receptor binding was lateralized.

Place, publisher, year, edition, pages
2014. , 43 p.
National Category
Pharmacology and Toxicology
URN: urn:nbn:se:uu:diva-231036OAI: oai:DiVA.org:uu-231036DiVA: diva2:742907
Subject / course
Pharmaceutical Pharmacology
Educational program
Master of Science Programme in Pharmacy
Available from: 2014-09-15 Created: 2014-09-02 Last updated: 2014-09-15Bibliographically approved

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