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An automated mass spectrometry-based screening method for analysis of sulfated glycosaminoglycans
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. Uppsala University, Science for Life Laboratory, SciLifeLab.
2014 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 450, no 1, 598-603 p.Article in journal (Refereed) Published
Abstract [en]

Glycosaminoglycans (GAGs) are linear polysaccharides, consisting of repeated disaccharide units, attached to core proteins in all multicellular organisms. Chondroitin sulfate (CS) and dermatan sulfate (DS) constitute a subgroup of sulfated GAGs for which the degree of sulfation varies between species and tissues. One major goal in GAG characterization is to correlate structure to function. A common approach is to exhaustively degrade the GAG chains and thereafter determine the amount of component disaccharide units. In large-scale studies, there is a need for high-throughput screening methods since existing methods are either very time- or samples consuming. Here, we present a new strategy applying MALDI-TOF MS in positive ion mode for semi-qualitative and quantitative analysis of CS/DS derived disaccharide units. Only a few picomoles of sample are required per analysis and 10 samples can be analyzed in 25 min, which makes this approach an attractive alternative to many established assay methods. The total CS/DS concentration in 19 samples derived from Caenorhabditis elegans and mammalian tissues and cells was determined. The obtained results were well in accordance with concentrations determined by a standard liquid chromatography-based method, demonstrating the applicability of the method for samples from various biological matrices containing CS/DS of different sulfation degrees.

Place, publisher, year, edition, pages
2014. Vol. 450, no 1, 598-603 p.
Keyword [en]
Mass spectrometry, Matrix-assisted laser desorption/ionization-time of flight mass spectrometry, Glycosaminoglycan, Chondroitin sulfate, Dermatan sulfate, Bioanalytical methods
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-231045DOI: 10.1016/j.bbrc.2014.06.011ISI: 000343641000098OAI: oai:DiVA.org:uu-231045DiVA: diva2:742934
Available from: 2014-09-03 Created: 2014-09-03 Last updated: 2017-12-05Bibliographically approved

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Publisher's full texthttp://www.sciencedirect.com/science/article/pii/S0006291X14010857

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Dierker, TabeaSpillmann, DorotheRamström, Margareta

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Analytical ChemistryScience for Life Laboratory, SciLifeLabDepartment of Medical Biochemistry and Microbiology
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