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In situ generation of iminodiacetic acid groups on nanoporous alumina for the reversible immobilization of enzymes and other biomolecules
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Biochemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Biochemistry.
2014 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 36, no 9, 1819-1825 p.Article in journal (Refereed) Published
Abstract [en]

Nanoporous alumina membranes were silanized with aminopropylsilane and iminodiacetic acid (IDA) groups were generated in situ by reaction with iodoacetate. The membranes were mounted in standard filter holders, connected to a HPLC system and saturated with selected metal ions. Cu(II) allowed the capture of chicken muscle lactate dehydrogenase with such stability, repeatability and reproducibility that Michaelis-Menten kinetics could be studied. The IDA surface was stable for months and could be depleted and regenerated with metal ions multiple times without appreciable loss of capacity. The binding of lactate dehydrogenase influenced the backpressure to the extent that could be expected for a monolayer according to Poiseuilles law.

Place, publisher, year, edition, pages
2014. Vol. 36, no 9, 1819-1825 p.
Keyword [en]
Enzyme reactor, Immobilized metal affinity chromatography, Iminodiacetic acid (IDA), Mass transport, Nanoporous alumina, Regeneration stability
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-230930DOI: 10.1007/s10529-014-1544-zISI: 000339937600010OAI: oai:DiVA.org:uu-230930DiVA: diva2:743567
Available from: 2014-09-04 Created: 2014-09-01 Last updated: 2017-12-05Bibliographically approved

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Kjellander, MarcusJohansson, Gunnar

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