uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Identification and characterization of a QM protein as a possible peptidoglycan recognition protein (PGRP) from the giant tiger shrimp Penaeus monodon
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
Show others and affiliations
2014 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 46, no 2, 146-154 p.Article in journal (Refereed) Published
Abstract [en]

In an attempt to identify a peptidoglycan recognition protein (PGRP) in Penaeus (Penaeus) monodon, in vitro pull-down binding assays were used between shrimp proteins and purified peptidoglycan (PG). By gel electrophoresis and mass spectrometry followed by Mascot program analysis, proteins from shrimp hemocyte peripheral membrane proteins showed significant homology to records for a QM protein, actin and prophenoloxidase 2 precursor (proPO2), while proteins from cell-free plasma showed significant homology to records for a vitellogenin, a fibrinogen related protein (FREP) and a C-type lectin. Due to time and resource limitations, specific binding to PG was examined only for recombinant PmQM protein and PmLec that were synthesized based on sequences reported in the Genbank database (accession numbers FJ766846 and DQ078266, respectively). An in vitro assay revealed that hemocytes would bind with and encapsulate agarose beads coated with recombinant PmQM (rPmQM) or rPmLec and that melanization followed 2 h post-encapsulation. ELISA tests confirmed specific binding of rPmQM protein to PG. This is the first time that PmQM has been reported as a potential PGRP in shrimp or any other crustacean. The two other potential PGRP identified (FREP and the vitellin-like protein present in male P. monodon, unlike other vitellin subunits) should also be expressed heterologously and tested for their ability to activate shrimp hemocytes.

Place, publisher, year, edition, pages
2014. Vol. 46, no 2, 146-154 p.
Keyword [en]
Peptidoglycan recognition proteins, Penaeus monodon, QM protein, PmQM, C-type lectin, PmLec
National Category
Zoology Immunology
URN: urn:nbn:se:uu:diva-230911DOI: 10.1016/j.dci.2014.04.003ISI: 000340216600004OAI: oai:DiVA.org:uu-230911DiVA: diva2:743610
Available from: 2014-09-04 Created: 2014-09-01 Last updated: 2014-09-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Junkunlo, Kingkamon
By organisation
Comparative Physiology
In the same journal
Developmental and Comparative Immunology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 204 hits
ReferencesLink to record
Permanent link

Direct link