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Proteome Analysis of Adenovirus Using Mass Spectrometry
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
2014 (English)In: Adenovirus: Methods and Protocols / [ed] Miguel Chillón, Assumpció Bosch, Springer Science+Business Media B.V., 2014, Vol. 1089, 25-44 p.Chapter in book (Refereed)
Abstract [en]

Analysis of proteins and their posttranslational modifications is important for understanding different biological events. For analysis of viral proteomes, an optimal protocol includes production of a highly purified virus that can be investigated with a high-resolving analytical method. In this Methods in Molecular Biology paper we describe a working strategy for how structural proteins in the Adenovirus particle can be studied using liquid chromatography–high-resolving mass spectrometry. This method provides information on the chemical composition of the virus particle. Further, knowledge about amino acids carrying modifications that could be essential for any part of the virus life cycle is collected. We describe in detail alternatives available for preparation of virus for proteome analysis as well as choice of mass spectrometric instrumentation suitable for this kind of analysis.

Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2014. Vol. 1089, 25-44 p.
Series
Methods in Molecular Biology, vol. 1089
Keyword [en]
Adenovirus, Proteome analysis, Posttranslational modification, Mass spectrometry, LC-MS
National Category
Microbiology Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-231187OAI: oai:DiVA.org:uu-231187DiVA: diva2:743797
Available from: 2014-09-05 Created: 2014-09-05 Last updated: 2014-09-05

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http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-62703-679-5_2

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Lind, Sara BergströmArtemenko, Konstantin A.

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