Analysis of l-[methyl-11C]methionine and metabolites in human plasma by an automated solid-phase extraction and a high-performance liquid chromatographic procedure
1996 (English)In: Journal of Chromatography B: Biomedical Sciences and Applications, ISSN 1387-2273, Vol. 679, no 1-2, 13-19 p.Article in journal (Refereed) Published
A fully automated method for separation of l-[methyl-11C]Methionine from metabolites in patient plasma was developed. l-[methyl-11C]Methionine was isolated from plasma by solid-phase extraction (SPE). The radioactivity retained on the SPE column was eluted and injected onto the HPLC system for separation of in vivo formed l-[methyl-11C]methionine radiolabeled metabolites. The yield through the isolation procedure and HPLC analysis was greater than 95% with a precision better than 5% (R.S.D.). The calculated rate of l-[methyl-11C]methionine transport into tumor tissue was markedly different with and without compensation for radiolabeled metabolises in patient plasma.
Place, publisher, year, edition, pages
1996. Vol. 679, no 1-2, 13-19 p.
IdentifiersURN: urn:nbn:se:uu:diva-231359DOI: 10.1016/0378-4347(96)00011-4PubMedID: 8998552OAI: oai:DiVA.org:uu-231359DiVA: diva2:744346