Characterization of Brown Adipose Tissue in the Human Perirenal Depot
2014 (English)In: Obesity, ISSN 1930-7381, E-ISSN 1930-739X, Vol. 22, no 8, 1830-1837 p.Article in journal (Refereed) Published
ObjectiveTo characterize brown adipose tissue (BAT) in the human perirenal adipose tissue depot. MethodPerirenal adipose tissue biopsies were obtained from 55 healthy kidney donors. Expression analysis was performed using microarray, real-time PCR, immunoblotting and immunohistochemistry. Additional studies using human stem cells were performed. ResultsUCP1 gene expression analysis revealed a large intra-individual variation in the perirenal adipose tissue biopsies. Both multi- and unilocular UCP1-positive adipocytes were detected in several of the adipose tissue samples analyzed by immunohistochemical staining. Microarray analysis identified 54 genes that were overexpressed in UCP1-positive perirenal adipose tissue. Real-time PCR analysis of BAT candidate genes revealed a set of genes that were highly correlated to UCP1 and a set of three transcription factor genes (PRDM16, PGC1, and RXR) that were highly correlated to each other. RXR displayed nuclear immunoreactivity in brown adipocytes and an increased gene expression during brown adipogenesis in human stem cells. ConclusionOur data provides the first molecular characterization of BAT in the perirenal adipose tissue depot. Furthermore, it highlights the transcription factor RXR as a new player in BAT development.
Place, publisher, year, edition, pages
2014. Vol. 22, no 8, 1830-1837 p.
Endocrinology and Diabetes Nutrition and Dietetics
IdentifiersURN: urn:nbn:se:uu:diva-231213DOI: 10.1002/oby.20765ISI: 000340295500011OAI: oai:DiVA.org:uu-231213DiVA: diva2:745096