uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Spatial control of membrane receptor function using ligand nanocalipers
Show others and affiliations
2014 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 11, no 8, 841-846 p.Article in journal (Refereed) Published
Abstract [en]

The spatial organization of membrane-bound ligands is thought to regulate receptor-mediated signaling. However, direct regulation of receptor function by nanoscale distribution of ligands has not yet been demonstrated, to our knowledge. We developed rationally designed DNA origami nanostructures modified with ligands at well-defined positions. Using these 'nanocalipers' to present ephrin ligands, we showed that the nanoscale spacing of ephrin-A5 directs the levels of EphA2 receptor activation in human breast cancer cells. Furthermore, we found that the nanoscale distribution of ephrin-A5 regulates the invasive properties of breast cancer cells. Our ligand nanocaliper approach has the potential to provide insight into the roles of ligand nanoscale spatial distribution in membrane receptor mediated signaling.

Place, publisher, year, edition, pages
2014. Vol. 11, no 8, 841-846 p.
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-231209DOI: 10.1038/NMETH.3025ISI: 000340075600024OAI: oai:DiVA.org:uu-231209DiVA: diva2:745116
Swedish Research Council, 2010-6296, 2010-5060
Available from: 2014-09-09 Created: 2014-09-05 Last updated: 2016-10-20

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Al-Amin, AbdullahBlokzijl, Andries
By organisation
Molecular tools
In the same journal
Nature Methods
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 198 hits
ReferencesLink to record
Permanent link

Direct link