Spatial control of membrane receptor function using ligand nanocalipers
2014 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 11, no 8, 841-846 p.Article in journal (Refereed) Published
The spatial organization of membrane-bound ligands is thought to regulate receptor-mediated signaling. However, direct regulation of receptor function by nanoscale distribution of ligands has not yet been demonstrated, to our knowledge. We developed rationally designed DNA origami nanostructures modified with ligands at well-defined positions. Using these 'nanocalipers' to present ephrin ligands, we showed that the nanoscale spacing of ephrin-A5 directs the levels of EphA2 receptor activation in human breast cancer cells. Furthermore, we found that the nanoscale distribution of ephrin-A5 regulates the invasive properties of breast cancer cells. Our ligand nanocaliper approach has the potential to provide insight into the roles of ligand nanoscale spatial distribution in membrane receptor mediated signaling.
Place, publisher, year, edition, pages
2014. Vol. 11, no 8, 841-846 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-231209DOI: 10.1038/NMETH.3025ISI: 000340075600024OAI: oai:DiVA.org:uu-231209DiVA: diva2:745116
FunderSwedish Research Council, 2010-6296, 2010-5060