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Lack of evidence for a role of islet autoimmunity in the aetiology of canine diabetes mellitus
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Autoimmunity. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Molecular epidemiology. Uppsala University, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-2071-5866
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Autoimmunity. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular and Morphological Pathology. Uppsala University, Science for Life Laboratory, SciLifeLab.
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2014 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, no 8, e105473- p.Article in journal (Refereed) Published
Abstract [en]

AIMS/HYPOTHESIS:

Diabetes mellitus is one of the most common endocrine disorders in dogs and is commonly proposed to be of autoimmune origin. Although the clinical presentation of human type 1 diabetes (T1D) and canine diabetes are similar, the aetiologies may differ. The aim of this study was to investigate if autoimmune aetiology resembling human T1D is as prevalent in dogs as previously reported.

METHODS:

Sera from 121 diabetic dogs representing 40 different breeds were tested for islet cell antibodies (ICA) and GAD65 autoantibodies (GADA) and compared with sera from 133 healthy dogs. ICA was detected by indirect immunofluorescence using both canine and human frozen sections. GADA was detected by in vitro transcription and translation (ITT) of human and canine GAD65, followed by immune precipitation. Sections of pancreata from five diabetic dogs and two control dogs were examined histopathologically including immunostaining for insulin, glucagon, somatostatin and pancreas polypeptide.

RESULTS:

None of the canine sera analysed tested positive for ICA on sections of frozen canine or human ICA pancreas. However, serum from one diabetic dog was weakly positive in the canine GADA assay and serum from one healthy dog was weakly positive in the human GADA assay. Histopathology showed marked degenerative changes in endocrine islets, including vacuolisation and variable loss of immune-staining for insulin. No sign of inflammation was noted.

CONCLUSIONS/INTERPRETATIONS:

Contrary to previous observations, based on results from tests for humoral autoreactivity towards islet proteins using four different assays, and histopathological examinations, we do not find any support for an islet autoimmune aetiology in canine diabetes mellitus.

Place, publisher, year, edition, pages
2014. Vol. 9, no 8, e105473- p.
National Category
Endocrinology and Diabetes
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URN: urn:nbn:se:uu:diva-232422DOI: 10.1371/journal.pone.0105473ISI: 000340952200053PubMedID: 25153886OAI: oai:DiVA.org:uu-232422DiVA: diva2:747935
Note

De två första författarna delar förstaförfattarskapet.

Available from: 2014-09-17 Created: 2014-09-17 Last updated: 2017-12-05Bibliographically approved

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Ahlgren, Kerstin MFall, ToveLandegren, NilsGrimelius, LarsLindblad-Toh, KerstinLobell, AnnaKämpe, Olle

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Ahlgren, Kerstin MFall, ToveLandegren, NilsGrimelius, LarsLindblad-Toh, KerstinLobell, AnnaKämpe, Olle
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AutoimmunityScience for Life Laboratory, SciLifeLabMolecular epidemiologyMolecular and Morphological PathologyGenomicsDepartment of Medical Sciences
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