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Single-layer centrifugation separates spermatozoa from diploid cells in epididymal samples from gray wolves, Canis lupus (L.)
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics.
2014 (English)In: Theriogenology, ISSN 0093-691X, E-ISSN 1879-3231, Vol. 82, no 5, 773-776 p.Article in journal (Refereed) Published
Abstract [en]

Sperm samples may be used for assisted reproductive technologies (e.g., farmed or endangered species) or as a source of haploid DNA or sperm-specific RNA. When ejaculated spermatozoa are not available or are very difficult to obtain, as is the case for most wild endangered species, the epididymides of dead animals (e.g., animals that have been found dead, shot by hunters or poachers, or that that require euthanasia in zoological collections) can be used as a source of sperm. Such epididymal sperm samples are usually contaminated with cellular debris, erythrocytes, leukocytes, and sometimes also bacteria. These contaminants may be sources of reactive oxygen species that damage spermatozoa during freezing or contribute undesired genetic material from diploid cells. We used single-layer centrifugation through a colloid formulation, Androcoll-C, to successfully separate wolf epididymal spermatozoa from contaminating cells and cellular debris in epididymal samples harvested from carcasses. Such a procedure may potentially be applied to epididymal sperm samples from other species.

Place, publisher, year, edition, pages
2014. Vol. 82, no 5, 773-776 p.
Keyword [en]
Androcoll-C, Assisted reproductive technologies, Colloid, Single-cell DNA, RNA, Sperm genotyping
National Category
Veterinary Science
Identifiers
URN: urn:nbn:se:uu:diva-232572DOI: 10.1016/j.theriogenology.2014.04.029ISI: 000340863200014OAI: oai:DiVA.org:uu-232572DiVA: diva2:749640
Available from: 2014-09-24 Created: 2014-09-22 Last updated: 2017-12-05Bibliographically approved

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