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Nanoflow electrospinning serial femtosecond crystallography
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2012 (English)In: Acta Crystallographica Section D: Biological Crystallography, ISSN 0907-4449, E-ISSN 1399-0047, Vol. 68, no Pt 11, 1584-1587 p.Article in journal (Refereed) Published
Abstract [en]

An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond 4 Å resolution, producing 14,000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.

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2012. Vol. 68, no Pt 11, 1584-1587 p.
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URN: urn:nbn:se:uu:diva-232842DOI: 10.1107/S0907444912038152PubMedID: 23090408OAI: oai:DiVA.org:uu-232842DiVA: diva2:749981
Available from: 2014-09-25 Created: 2014-09-25 Last updated: 2014-10-08Bibliographically approved

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Seibert, M Marvin
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