Missense substitutions lethal to essential functions of EF-Tu
1991 (English)In: Biochimie, ISSN 0300-9084, E-ISSN 1638-6183, Vol. 73, no 12, 1457-1464 p.Article in journal (Refereed) Published
We have used a simple selection and screening method to isolate function defective mutants of EF-Tu. From 28 mutants tested, 12 different missense substitutions, individually lethal to some essential function of EF-Tu, were identified by sequencing. In addition we found a new non-lethal missense mutation. The frequency of isolation of unique mutations suggests that this method can be used to easily isolate many more. The lethal mutations occur in all three structural domains of EF-Tu, but most are in domain II. We aim to use these mutants to define functional domains on EF-Tu.
Place, publisher, year, edition, pages
1991. Vol. 73, no 12, 1457-1464 p.
Microbiology in the medical area
IdentifiersURN: urn:nbn:se:uu:diva-47258DOI: 10.1016/0300-9084(91)90178-4OAI: oai:DiVA.org:uu-47258DiVA: diva2:75165