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Mutant ribosomes can generate dominant kirromycin resistance
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Molecular Biology.
1991 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 173, no 12, 3635-3643 p.Article in journal (Refereed) Published
Abstract [en]

Mutations in the two genes for EF-Tu in Salmonella typhimuriumand Escherichia coli, tufA and tufB, can confer resistance tothe antibiotic kirromycin. Kirromycin resistance is a recessivephenotype expressed when both tuf genes are mutant. We describea new kirromycin-resistant phenotype dominant to the effectof wild-type EF-Tu. Strains carrying a single kirromycin-resistanttuf mutation and an error-restrictive, streptomycin-resistantrpsL mutation are resistant to high levels of kirromycin, evenwhen the other tuf gene is wild type. This phenotype is dependenton error-restrictive mutations and is not expressed with nonrestrictivestreptomycin-resistant mutations. Kirromycin resistance is alsoexpressed at a low level in the absence of any mutant EF-Tu.These novel phenotypes exist as a result of differences in theinteractions of mutant and wild-type EF-Tu with the mutant ribosomes.The restrictive ribosomes have a relatively poor interactionwith wild-type EF-Tu and are thus more easily saturated withmutant kirromycin-resistant EF-Tu. In addition, the mutant ribosomesare inherently kirromycin resistant and support a significantlyfaster EF-Tu cycle time in the presence of the antibiotic thando wild-type ribosomes. A second phenotype associated with combinationsof rpsL and error-prone tuf mutations is a reduction in thelevel of resistance to streptomycin.

Place, publisher, year, edition, pages
1991. Vol. 173, no 12, 3635-3643 p.
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-47264OAI: oai:DiVA.org:uu-47264DiVA: diva2:75171
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2017-12-05Bibliographically approved

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http://jb.asm.org/cgi/content/short/173/12/3635

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Hughes, Diarmaid

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