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The use of multiplex platforms for absolute and relative protein quantification of clinical material
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Haematology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Haematology.
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2014 (English)In: EuPA Open Proteomics, ISSN 2212-9685, Vol. 3, 37-47 p.Article in journal (Refereed) Published
Abstract [en]

When introducing multiplex platforms to measure protein content in precious clinical material there is an increased risk of cross reactivity, loss of sensitivity as well as accuracy. In this paper, four multiplex platforms and one singleplex platform were compared by running pre- and post-treatment plasma samples from CML patients. We found a variation of absolute protein concentrations between platforms. For some of the analytes and platforms, relative differences between pre- and post-treatment samples correlated. We conclude that absolute concentrations measured by different platforms should be compared with caution and comparing relative differences could be more accurate.

Place, publisher, year, edition, pages
2014. Vol. 3, 37-47 p.
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Clinical Medicine
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URN: urn:nbn:se:uu:diva-233828DOI: 10.1016/j.euprot.2014.02.002OAI: oai:DiVA.org:uu-233828DiVA: diva2:754459
Available from: 2014-10-10 Created: 2014-10-10 Last updated: 2015-04-10Bibliographically approved

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Simonsson, BengtOlsson-Strömberg, UllaLoskog, Angelica S.I.Mangsbo, Sara

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Simonsson, BengtOlsson-Strömberg, UllaLoskog, Angelica S.I.Mangsbo, Sara
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Department of Immunology, Genetics and PathologyScience for Life Laboratory, SciLifeLabHaematologyClinical Immunology
Clinical Medicine

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