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Engineering of a bispecific affibody molecule towards HER2 and HER3 by addition of an albumin-binding domain allows for affinity purification and in vivo half-life extension
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2014 (English)In: Biotechnology Journal, ISSN 1860-6768, Vol. 9, no 9, 1215-1222 p.Article in journal (Refereed) Published
Abstract [en]

Emerging strategies in cancer biotherapy include the generation and application of bispecific antibodies, targeting two tumor-associated antigens for improved tumor selectivity and potency. Here, an alternative format for bispecific molecules was designed and investigated, in which two Affibody molecules were linked by an albumin-binding domain (ABD). Affibody molecules are small (6 kDa) affinity proteins and this new format allows for engineering of molecules with similar function as full-length bispecific antibodies, but in a dramatically smaller size (around eight-fold smaller). The ABD was intended to function both as a tag for affinity purification as well as for in vivo half-life extension in future preclinical and clinical investigations. Affinity-purified bispecific Affibody molecules, targeting HER2 and HER3, showed simultaneous binding to the three target proteins (HER2, HER3, and albumin) when investigated in biosensor assays. Moreover, simultaneous interactions with the receptors and albumin were demonstrated using flow cytometry on cancer cells. The bispecific Affibody molecules were also able to block ligand-induced phosphorylation of the HER receptors, indicating an anti-proliferative effect. We believe that this compact and flexible format has great potential for developing new potent bispecific affinity proteins in the future, as it combines the benefits of a small size (e.g. improved tissue penetration and reduced cost of goods) with a long circulatory half-life.

Place, publisher, year, edition, pages
2014. Vol. 9, no 9, 1215-1222 p.
Keyword [en]
Affibody molecules, Albumin-binding domain, Bispecific, Half-life extension, HER3
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-234159DOI: 10.1002/biot.201400009ISI: 000341694200013OAI: oai:DiVA.org:uu-234159DiVA: diva2:755856
Available from: 2014-10-15 Created: 2014-10-14 Last updated: 2014-10-15Bibliographically approved

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Frejd, Fredrik Y.
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Biomedical Radiation Sciences
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