uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
High prevalence of Plasmodium falciparum pfcrt K76T mutation in children with sickle cell disease at a tertiary hospital in north-western Tanzania
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Show others and affiliations
2014 (English)In: Tanzania Journal of Health Research, ISSN 1821-6404, Vol. 16, no 4Article in journal (Refereed) Published
Abstract [en]

The high prevalence of sickle cell disease (SCD) and trait in Sub-Saharan Africa coincides with thedistribution of Plasmodium falciparum malaria. Due to prolonged heavy use of chloroquine (CQ) as anantimalarial, drug resistance has developed. Many countries including Tanzania abandoned the use of CQfor uncomplicated malaria, except its use as prophylaxis in patients with sickle cell disease. This studyinvestigated the prevalence of malaria in SCD patients and mutations associated with CQ resistance.Children diagnosed with sickle cell disease attending both outpatient clinic and those admitted at BugandoMedical Centre in north-western Tanzania were screened for malaria using thick blood smear. A driedblood spot on Whatman filter paper was also taken for polymerase chain reaction (PCR) and restrictionfragment length polymorphism. Among 123 known patients with sickle cell disease, the prevalence ofmalaria by blood smear microscopy was 3.2% and by PCR was 13.8%. The prevalence of K76T mutationamong the patients was 81.3%. The majority of the patients (72.4%) were using chloroquine prophylaxis. Inconclusion, the prevalence of malaria parasitaemia among children with sickle cell disease attending BMC islow (3.2%) by microscopy but several children maintain sub patent infection detectable by PCR. Theprevalence of chloroquine resistant P. falciparum in these children was higher than that previously seen innormal population in Tanzania. We recommend special attention to be paid to patients with sickle celldisease while studying the dynamics of drug resistant parasites.

Place, publisher, year, edition, pages
2014. Vol. 16, no 4
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:uu:diva-235231DOI: 10.4314/thrb.v16i4.1OAI: oai:DiVA.org:uu-235231DiVA: diva2:759411
Available from: 2014-10-29 Created: 2014-10-29 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

Sickle cell(143 kB)302 downloads
File information
File name FULLTEXT01.pdfFile size 143 kBChecksum SHA-512
0424049b12455e073f2d4c5aee284f5c2e164b3720644da81a078e30e501bfd516939beb2257343a349d6edf13c07047bb3dcb345b2886cafa41ff25bb15d1db
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Enweji, NizarSwedberg, Göte

Search in DiVA

By author/editor
Enweji, NizarSwedberg, Göte
By organisation
Department of Medical Biochemistry and Microbiology
In the same journal
Tanzania Journal of Health Research
Clinical Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 302 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 712 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf