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High prevalence of Plasmodium falciparum pfcrt K76T mutation in children with sickle cell disease at a tertiary hospital in north-western Tanzania
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2014 (English)In: Tanzania Journal of Health Research, ISSN 1821-6404, Vol. 16, no 4Article in journal (Refereed) Published
Abstract [en]

The high prevalence of sickle cell disease (SCD) and trait in Sub-Saharan Africa coincides with thedistribution of Plasmodium falciparum malaria. Due to prolonged heavy use of chloroquine (CQ) as anantimalarial, drug resistance has developed. Many countries including Tanzania abandoned the use of CQfor uncomplicated malaria, except its use as prophylaxis in patients with sickle cell disease. This studyinvestigated the prevalence of malaria in SCD patients and mutations associated with CQ resistance.Children diagnosed with sickle cell disease attending both outpatient clinic and those admitted at BugandoMedical Centre in north-western Tanzania were screened for malaria using thick blood smear. A driedblood spot on Whatman filter paper was also taken for polymerase chain reaction (PCR) and restrictionfragment length polymorphism. Among 123 known patients with sickle cell disease, the prevalence ofmalaria by blood smear microscopy was 3.2% and by PCR was 13.8%. The prevalence of K76T mutationamong the patients was 81.3%. The majority of the patients (72.4%) were using chloroquine prophylaxis. Inconclusion, the prevalence of malaria parasitaemia among children with sickle cell disease attending BMC islow (3.2%) by microscopy but several children maintain sub patent infection detectable by PCR. Theprevalence of chloroquine resistant P. falciparum in these children was higher than that previously seen innormal population in Tanzania. We recommend special attention to be paid to patients with sickle celldisease while studying the dynamics of drug resistant parasites.

Place, publisher, year, edition, pages
2014. Vol. 16, no 4
National Category
Clinical Medicine
URN: urn:nbn:se:uu:diva-235231DOI: 10.4314/thrb.v16i4.1OAI: oai:DiVA.org:uu-235231DiVA: diva2:759411
Available from: 2014-10-29 Created: 2014-10-29 Last updated: 2014-10-30Bibliographically approved

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Enweji, NizarSwedberg, Göte
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