uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
In situ sequencing identifies TMPRSS2-ERG fusion transcripts, somatic point mutations and gene expression levels in prostate cancers
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Genomics. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Genomics.
Show others and affiliations
2014 (English)In: Journal of Pathology, ISSN 0022-3417, E-ISSN 1096-9896, Vol. 234, no 2, 253-261 p.Article in journal (Refereed) Published
Abstract [en]

Translocations contribute to the genesis and progression of epithelial tumours and in particular to prostate cancerdevelopment. To better understand the contribution of fusion transcripts and visualize the clonal composition ofmultifocal tumours, we have developed a technology for multiplexin situdetection and identification of expressedfusion transcripts. When compared to immunohistochemistry,TMPRSS2–ERGfusion-negative and fusion-positiveprostate tumours were correctly classified. The most prevalentTMPRSS2–ERGfusion variants were visualized,identified, and quantitated in human prostate cancer tissues, and the ratio of the variant fusion transcripts couldfor the first time be directly determined byin situsequencing. Further, we demonstrate concurrentin situdetectionof gene expression, point mutations, and gene fusions of the prostate cancer relevant targetsAMACR,AR,TP53,andTMPRSS2–ERG. This unified approach toin situanalyses of somatic mutations can empower studies ofintra-tumoural heterogeneity and future tissue-based diagnostics of mutations and translocations.

Place, publisher, year, edition, pages
2014. Vol. 234, no 2, 253-261 p.
National Category
Medical Genetics
Identifiers
URN: urn:nbn:se:uu:diva-235568DOI: 10.1002/path.4392ISI: 000342976700013PubMedID: 24931216OAI: oai:DiVA.org:uu-235568DiVA: diva2:761238
Note

De två första författarna delar förstaförfattarskapet.

Available from: 2014-11-06 Created: 2014-11-06 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Kiflemariam, SaraAli, Muhammad AkhtarSjöblom, Tobias

Search in DiVA

By author/editor
Kiflemariam, SaraAli, Muhammad AkhtarSjöblom, Tobias
By organisation
GenomicsScience for Life Laboratory, SciLifeLab
In the same journal
Journal of Pathology
Medical Genetics

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 693 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf