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A novel mass spectrometric approach to analysis of hormonal peptides in extracts of mouse pancreatic islets
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
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2003 (English)In: European Journal of Biochemistry, ISSN 0014-2956, E-ISSN 1432-1033, Vol. 270, no 15, 3146-3152 p.Article in journal (Refereed) Published
Abstract [en]

Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e. nozzle-skimmer fragmentation and electron capture dissociation, for the determination of hormonal peptides in an acid ethanol extract of mouse pancreatic islets. The most abundant peptides, those derived from proinsulin and proglucagon, were identified by their masses and additional sequence-tag information established their identities. Interestingly, the experiments demonstrated the presence of truncated C-peptides, des-(25-29)-C-peptide and des-(27-31)-C-peptide. These novel findings clearly illustrate the potential usefulness of the described technique for on-line sequencing and characterization of peptides in tissue extracts.

Place, publisher, year, edition, pages
2003. Vol. 270, no 15, 3146-3152 p.
Keyword [en]
liquid chromatography, Fourier transform ion cyclotron resonance mass spectrometry, electron capture dissociation, pancreas, peptides
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-48498DOI: 10.1046/j.1432-1033.2003.03690.xPubMedID: 12869189OAI: oai:DiVA.org:uu-48498DiVA: diva2:76404
Available from: 2007-04-19 Created: 2007-04-19 Last updated: 2017-12-05

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Ramström, MargaretaHåkansson, PerBergquist, Jonas

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