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Separation and purification of puerarin using beta-cyclodextrin-coupled agarose gel media
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
2004 (English)In: J. Chromatogr. A, Vol. 1022, no 1-2, 77-82 p.Article in journal (Refereed) Published
Abstract [en]

The isoflavonoid puerarin, a well-known traditional Chinese drug, has been purified in one step from an extract of Radix puerariae (root of the plant Pueraria lobata) by adsorption chromatography on an epichlorohydrin polymerized beta-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP. Acetic acid (10%) was used as the mobile phase and the optimum loading capacity was around 1.2 mg crude extract/ml packed gel. The purity of the collected puerarin was about 98% with a recovery of about 62%.

Place, publisher, year, edition, pages
2004. Vol. 1022, no 1-2, 77-82 p.
Keyword [en]
stationary phases, LC, pharmaceutical analysis, puerarin, cyclodextrin, agarose, isoflavonoids
Identifiers
URN: urn:nbn:se:uu:diva-48554OAI: oai:DiVA.org:uu-48554DiVA: diva2:76460
Available from: 2007-02-14 Created: 2007-02-14 Last updated: 2011-01-12

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