uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Anakinra and Tocilizumab Enhance Survival and Function of Human Islets During Culture: Implications for Clinical Islet Transplantation
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
Show others and affiliations
2014 (English)In: Cell Transplantation, ISSN 0963-6897, E-ISSN 1555-3892, Vol. 23, no 10, 1199-1211 p.Article in journal (Refereed) Published
Abstract [en]

Pretreatment culture before islet transplantation represents a window of opportunity to ameliorate the pro-inflammatory profile expressed by human beta-cells in duress. Analcinra (IL-1 receptor antagonist) and tocilizumab (monoclonal IL-6 receptor antibody) are two known anti-inflammatory agents successfully used in the treatment of inflammatory states like rheumatoid arthritis. Both compounds have also been shown to reduce blood glucose and glycosylated hemoglobin in diabetic patients. We therefore sought to evaluate the impact of anakinra and tocilizumab on human p-cells. The islets were precultured with or without anakinra or tocilizumab and then transplanted in a marginal mass model using human islets in immunodeficient mice. Islet viability was evaluated in an in vitro model. The pretreatment culture led to a significantly improved engraftment in treated islets compared to the vehicle. Anakinra and tocilizumab are not toxic to human islets and significantly reduce markers of inflammation and cell death. These results strongly support a pretreatment culture with anakinra and tocilizumab prior to human islet transplantation.

Place, publisher, year, edition, pages
2014. Vol. 23, no 10, 1199-1211 p.
Keyword [en]
Anakinra, Tocilizumab, Islet, Transplantation, Culture
National Category
Cell and Molecular Biology Immunology in the medical area
URN: urn:nbn:se:uu:diva-237638DOI: 10.3727/096368913X667529ISI: 000343381500003OAI: oai:DiVA.org:uu-237638DiVA: diva2:768603
Available from: 2014-12-04 Created: 2014-12-03 Last updated: 2014-12-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Clinical Immunology
In the same journal
Cell Transplantation
Cell and Molecular BiologyImmunology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 212 hits
ReferencesLink to record
Permanent link

Direct link